When you’re part of the team at Thermo Fisher Scientific, you’ll do important work, like helping customers in finding cures for cancer, protecting the environment or making sure our food is safe. Your work will have real-world impact, and you’ll be supported in achieving your career goals.
Be part of the genome engineering revolution. This scientist will develop tools and techniques to support genome engineering. This scientist will be expected to work in a highly collaborative team environment, supporting some projects with some supervision of a senior scientist and leading some projects independently. Projects may include, but not be limited to, cloning, purification and characterization of proteins, developing gene editing strategies in various cell lines, and developing assays to monitor editing.
Scope of Need
The successful individual will directly interact with senior R&D scientists and project management to execute project work plans. The scientist will be responsible for supporting or executing these plans to meet agreed upon deliverables and timelines. These activities will require a high level of independence as well as the ability to work in a highly cooperative team.
This role will involve the following tasks:
- Molecular biology
- Plasmid subcloning, DNA preparations, sequencing, in vitro transcription of RNA, PCR
- Generation of genome engineering tools
- Protein purification (AKTA)
- Protein characterization assay
- Tissue culture (Culture of many cell types)
- Genomic editing (TALN and CRISPR based editing, design, and implementation)
- Stable cell line generation (Transfection, transduction, antibiotic-selection, clonal expansion)
This individual will also be expected to support and participate in R&D and project management meetings.
Capabilities and Competencies
This position requires the following capabilities and competencies:
- Perform standard laboratory math calculations and solutions/media preparation
- Proven experience:
- Protein purification (preferably with experience with AKTA)
- Protein characterization (assay development)
- Genome engineering (CRISPR or TALEN)
- Ability to:
- Observe deviations from the expected outcomes and trouble-shoot
- Succeed with a moderate level of supervision and guidance
- Participate in a strong teamwork orientation with demonstrable influence/interpersonal skills
- Maintain a detailed lab notebook and present your findings in a group setting
The Program Manager will interact with internal employees and management, as well as third-party providers, and customers.
The vast majority of working time will be spent in a laboratory environment. Cell work will take place in biosafety hoods in a tissue culture laboratory, working with various cell cultures. Time may be spent manipulating DNA constructs and proteins in a laboratory environment. Some time will be spent at the desk recording laboratory activities appropriately in notebooks.
Candidate must be capable of observing and adhering to lab safety standards and protocols.
This individual must be able to identify deviations from expected outcomes and participate in troubleshooting those deviations and suggesting alternatives.
Critical to success in this position will be the individual’s ability to work independently on some projects and to provide support collaboratively on others while meeting project timelines.
Equally important will be the ability to work as part of a team in the laboratory (extended R&D team) as well as a broader cross-functional team (R&D, Project Management, Collaborator).
- Bachelor’s degree or a master’s degree may be considered if they have a significant amount of the appropriate experience.
- 3+ years of experience in molecular biology techniques (DNA/RNA/protein)
- 3+years of protein characterization and purification
- 1+ years of experience in stable cell line generation
- 1+ year experience with genome engineering of mammalian cells
- Experience with NGS
- Experience with FACS