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Job ID :
54242BR
Location :
India - Bangalore
:
Job Description

Responsibilities

  • Performing cell culture activities and laboratory techniques including aseptic expansion of mammalian cell lines, handling primary cells and immortal cells.
  • Routine transfections, high throughput transfections and mycoplasma testing
  • Initiate, plan and execute scientific research with emphasis on protein or DNA delivery methods (transformation, electroporation, transfection or transduction).
  • High-throughput molecular cloning using recombinant DNA technologies
  • Molecular cloning (include ligation independent methods), plasmid vector construction, DNA/RNA modification enzyme use, mutagenesis, selection systems), genome editing.
  • Independently complete experiments using these methodologies - PCR Purification, Restriction, Ligation, Transformation, Miniprep, Colony PCR, Sequencing
  • Keeping thorough record of cell culture parameters and results into data sheets and/or electronic note books.
  • Responsible for data integrity and accuracy.
  • Other functions such as writing technical protocols and filling out order forms.
  • Deliver high quality results according to project schedule in support of product commercialization during early feasibility, development and validation phases.

Skills

  • Should have a clear understanding of recombinant DNA technologies
  • Should have a clear understanding of different molecular cloning and gene engineering techniques
  • Knows therory, has full command of gene engineering methods (cloning methods (include ligation independent), plasmid vector construction, DNA/RNA modification enzyme use, mutagenesis, selection systems), genome editing.
  • Strong understanding and hands-on experience of cell-culture and DNA delivery methods
  • Should have a clear understanding and laboratory skills in the area of immunoassays like western blot, ELISA, and immunofluorescence
  • GLP experience, documentation and automation
  • Publications in peer-reviewed journals would be a bonus. Good understanding of research projects using standard techniques and protocols.
  • Should be a good team player, ready to learn new techniques and innovative thinker.
  • Industry experience, bioinformatics and grant writing skills will be considered as a bonus.
  • Excellent organizational skills with attention to detail.


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